BL21(DE3)pLysS Competent Cells, Promega

SKU: L1191
Brand: Promega Corporation
Product Code: 00002-27299
SKU: L1191
Availability: In Stock
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Important - Due to the nature of this product, all orders must be shipped to verifiable businesses at commercial addresses.

BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site.

  • Inducible Protein Expression
  • Increased Stability of Expressed Protein
  • Lower Background Expression of Target Genes
  • Genotype: F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, Cmʳ
BL21(DE3)pLysS is lysogenic for λ-DE3, which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter. BL21(DE3)pLysS also contains a plasmid, pLysS, which carries the gene encoding T7 lysozyme. T7 lysozyme lowers the background expression level of target genes under the control of the T7 promoter but does not interfere with the level of expression achieved following induction by IPTG.

For researchers doing more than one transformation, competent cells are available in standard format (200 µL aliquots). For added convenience, single-use competent cells (50 µL aliquots) also are offered.


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